Updated: Oct 11, 2020
Student of UIET, Panjab University
My internship experience at IISER Mohali, in short, has been ‘Career Defining'.
I got an opportunity to work under the guidance of Dr. Sharvan Sehrawat. The main focus of his work is the Induction of adaptive immune response and its maintenance in the memory phase forms the basis of lasting protective immunity against infectious diseases and provides a clue for successful vaccination.
After receiving help from CD4 T cells, pathogen-specific CD8 T cells are appropriately activated to control the spread of intracellular pathogens such as viruses. My project was to study the G-protein coupled receptor and to purify it using Nickel NTA in beads.G-protein-coupled receptor is a protein encoded by ADGRG5 gene. It is a member of the adhesion GPCR family. GPR114 mRNA is specifically expressed in human eosinophils as well as in mouse lymphocytes, monocytes, macrophage, and dendritic cells. They may play a role in the immune system as well as in the central nervous system. In the Ni-NTA separation method, it involves the use of peptide affinity tags, which are fused to the protein of interest and used to expedite protein purification via affinity chromatography.
The overall experience of this internship was very enriching.